OUR VISION

Ethical approach, strong structure, supportive context and a shared mindset are the strengths of the highly integrated Alchilife research team.
Specialized in the ophthalmic field with great expertise in chemistry, biology, biotechnology and bioengineering, the Alchimia R&D department cares to safeguard the patient’s health.

NEW PROJECTS

Cornea Surgery protectants

High viscosity fluid to protect the corneal surface and enhance cataract and vitreoretinal surgery performance.

Retina surgery staining

Reversible dye for retinal staining.

Vitreous visualization

Innovative device for vitreous visualization and removal during vitrectomy.

Retinal surgery instruments

Disposable vitreoretinal instruments for specific precision procedures.

Cryoprotectants

For human cell and tissue cryopreservation.

Innovative storage media

For donor cornea intended for transplantation without animal derivatives.

Post-surgery eye drops

EXPERTISE

Cytotoxicity tests in vitro
Microbiological analysis
Physico-chemical analysis
Experimental ex-vivo models
Wet lab sessions
Materials selection
Medical devices validation
Data and statistical analysis

SCIENTIFIC COLLABORATIONS

Dr Jean-Marie Parel, Ophthalmic Biophysics Center of the Bascom Palmer Eye Institute, University of Miami Miller Medical School
Universitätsgewebebank– Mikrobiologisches Kontrollabor Berlin, Germany
Centre for Cell, Gene & Tissue Therapeutics at Royal Free Hospital London, UK
Centro de Oftalmología Barraquer, Barcelona, Spain
Eye Bank of Rome, Azienda Ospedaliera S. Giovanni Addolorata, Italy
Monza Eye Bank, Italy
Banca Tessuti Cardiovascolari Regione Lombardia c/o Centro Cardiologico Monzino, Milano, Italy
Banca dei tessuti cardiovascolari della Regione Emilia Romagna, Italy
Istituto Clinico S. Camillo, Unità Operativa di Oculistica, Brescia, Italy
Polivisus, Diagnostica e Microchirurgia Oculare, Brescia, Italy
Banca della cute di Verona, Italy
Mikhail V Tsurkan PhD, Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden
Fondazione Banca degli Occhi del Veneto (FBOV)
Rita Mencucci, Careggi, Firenze, Italy

The latest R&D project

OPHTHALMIC DIAGNOSTICS

A transparent and sterile coupling gel to be used as an interface between the diagnostic tool and the corneal surface.
OCIGEL is the last R&D project specially designed for the hospital environment and medical ambulatory.
The product is available on the market with the CE mark.

ON MARKET

RINSING

BASE

A rinsing solution for tissues and cells intended for transplantation

DECONTAMINATION

BASE.128

An antibiotic cocktail for effective decontamination of human tissue intended for transplantation

CRYOPRESERVATION

CRYO.ON

100% dimethyl sulfoxide (DMSO) with purity higher than 99,9% which protects human tissues and cells during storage at cryogenic temperatures

CRYOPRESERVATION

GLYO.ON

A pure glycerol for use with tissues and cells intended for transplantation

OPHTHALMIC GEL

eyeDRO

A high viscosity fluid to protect the corneal surface and enhance cataract and vitreoretinal surgery performance

DYES FOR POSTERIOR SEGMENT

TWIN

A dye for ILM and pre retinal membrane staining

DYES FOR POSTERIOR SEGMENT

viewILM

A dye for the ILM staining

MICROBIOLOGY

RESEP

An innovative in vitro diagnostic medical device, designed to remove antibiotic residues from liquid samples undergoing microbiological analyses

METHODS

Validation of a semi-automated quantitative assessment of endothelial cell mortality using the Fiji Imaj software system

The research involved in the field of ophthalmology, especially when focused in testing and in […]

Validation of a sterility test of corneal storage media in compliance with the European Pharmacopoeia for a truly reliable screening: the experience of an Italian eye bank

It is well-known that cornea transplantation is still associated with a certain – though quite […]

Validation of a cytotoxicity test on vital dyes for anterior segment ophthalmic surgery according to the ISO 10993-5

When the visualization of the anterior capsule is compromised due to absent or insufficient red […]

Truly safe vital dye for posterior segment eye membranes: a reliable assistant in vitreoretinal surgery

During vitreoretinal procedures, the visualization of transparent structures such as the vitreous cortex, ERM, and […]

Finding the Minimum Inhibitory Concentrations of Amphotericin B for various strains of Candida

In the United States of America, corneal tissues from deceased donors are stored in cold […]

Antimycotic efficacy of a new Amphotericin B-containing cold corneal storage medium by time-kill studies: results and clinical implications

The new lamellar techniques for selective replacement of diseased layers of the cornea, which are […]

Perfluorocarbons for intraocular use: validation of a cytotoxicity test according to the ISO 10993-5

The Alchilife R& team, in collaboration with Mario Romano, MD, is one of the first […]

Intraocular medical devices: setup and fine-tuning of truly reliable cytotoxicity tests

The Alchilife R&D team is engaged in tuning-up truly reliable cytotoxicity tests performed according […]

SCIENTIFIC
HIGHLIGHTS

PUBLICATIONS

Comparison of Perfluorocarbon Liquids Cytotoxicity Tests: Direct Contact Versus the Test on Liquid Extracts
Year: 2022 Authors: Gatto C.; Ruzza P.; Giurgola L.; Honisch C.; Rossi O.; Romano M. R.; Ragazzi E.; D'Amato Tóthová J. ACS Omega Journal doi/10.1021/acsomega.2c04697 Online version This is a: Publication The purpose of this study is to compare the in vitro cytotoxicity tests according to the ISO 10993-5 (2009) standards using direct [...]
Killing efficacy of a new hypothermic corneal storage medium against the micro-organisms frequently found in human donor cornea intended for transplantation
Year: 2021 Authors: Giurgola L.; Gatto C.; Honisch C.; Rossi O.; Ragazzi E.; D'Amato Tóthová J. BMJ Open Ophthalmology 10.1136/bmjophth-2021-000833 Online version This is a: Publication Objective: To study the in vitro killing efficacy of Kerasave (AL.CHI.MI.A Srl), a medium provided with amphotericin B tablet for hypothermic storage of human donor corneas, against [...]
Ex vivo evaluation of retinal cytotoxicity after the use of multiple medical devices in pars plana vitrectomy in porcine eyes
Year: 2021 Authors: Gatto C.; Romano M.R.; Giurgola L.; Ferrara M.; Ragazzi E.; D'Amato Tóthová J. Experimental Eye Research 10.1016/j.exer.2021.108837 Online version This is a: Publication This study aimed to evaluate viability of retinal cells after the use of multiple intraoperative devices, namely a vitreal dye (triamcinolone acetonide,TA), a ERM/ILM dye (solution of trypan [...]
Toxicity Threshold of Perfluorocarbon Liquids for Intraocular Use: Dose–Response Assessment of In Vitro Cytotoxicity of Possible Contaminants
Year: 2021 Authors: Romano M.; Gatto C.; Giurgola L.; Ragazzi E.; D'Amato Tóthová J. Translational Vision Science & Technology 10.1167/tvst.10.6.24 Online version This is a: Publication Purpose: This study assessed the cytotoxicity of the impurities detected in the perfluorooctane (PFO) batches for vitreoretinal surgery that were associated with serious adverse incidents of ocular toxicity, [...]
Intraoperative efficacy and clinical outcomes of two commercial staining solutions used in idiopathic epiretinal membrane surgery
Year: 2021 Authors: Iuliano L.; Kacerik E.; Corbelli E.; Bandello F.; Codenotti M. Int Ophthalmol, 2021 doi.org/10.1007/s10792-020-01660-6 Online version This is a: Publication Purpose: To compare two commercially available staining solutions (MembraneBlue Dual® by D.O.R.C., Netherlands, and TWIN by AL.CHI.MI.A. S.R.L., Italy), in terms of intraoperative handling, staining efficacy and safety, in eyes [...]
A new storage medium containing amphotericin B versus Optisol-GS for preservation of human donor corneas
Year: 2020 Authors: Mistò R.; Giurgola L.; Pateri F.; Limongelli A.; Ragazzi E.; D’Amato Tóthová J. British Journal of Ophthalmology 2020, Nov 10 doi: 10.1136/bjophthalmol-2020-317136 Online version This is a: Publication Background/Aim: We compared the quality of human donor corneas stored in a cold storage medium containing 2.5 μg/ml of amphotericin B (Kerasave, AL.CHI.MI.A. S.R.L., Ponte San Nicolò, Italy) [...]
Safety of silicone oils as intraocular medical device: An in vitro cytotoxicity study
Year: 2020 Authors: Romano M. R.; Ferrara M.; Gatto C.; Giurgola L.; Zanoni M.; Angi M.; Rinaldi M.; Borgia A.; Sorrentino T.; D'Amato Tóthová J. Experimental Eye Research: May 2020 doi: 10.1016/j.exer.2020.108018 Online version This is a: Publication This study aimed to assess the cytotoxic effect of low molecular weight components (LMWC) and conventional [...]
Performance of New Hypothermic Corneal Storage Media With an Antimycotic Tablet in Comparison to Traditional Hypothermic Media During Simulated Eye Bank Processing
Year: 2020 Authors: Perry I.; Peterson K.; D'Amato Tóthová J.; Tramber M.; Botsay S.; Tremblay D. Cornea: April 13, 2020 - Volume Publish Ahead of Print doi: 10.1097/ICO.0000000000002333 Online version This is a: Publication Purpose: To compare the performance of Kerasave (AL.CHI.MI.A. S.R.L., Ponte San Nicolò, Italy) containing 2.5 μg/mL of amphotericin B and Optisol-GS (Bausch & Lomb, Bridgewater, [...]
Application of Circular Dichroism and Fluorescence Spectroscopies To Assess Photostability of Water-Soluble Porcine Lens Proteins
Year: 2020 Authors: Honisch C; Donadello V.; Rohanah H.; Peterle D.; De Filippis V.; Arrigoni G.; Gatto C.; Giurgola L.; Siligardi G.; Ruzza P. ACS Omega. 2020 Mar 3; 5(8): 4293–4301. doi: 10.1021/acsomega.9b04234 Online version This is a: Publication Abstract: The eye lens is mainly composed of the highly ordered water-soluble (WS) proteins named crystallins. [...]
A new approach to extend the storage of donor corneas after 28 days of corneal culture in an Italian eye bank
Year: 2019 Authors: Mistò R.; D’Amato Tóthová J.; Khan S.; Limongelli A.; Pateri F. Cell Tissue Bank: 2020 Mar;21(1):47-55 doi: 10.1007/s10561-019-09795-2 Online version This is a: Publication This study aimed to evaluate the possibility to extend the storage of unused organ-cultured donor corneas. After 28 days of corneal culture in TISSUE-C (AL.CHI.MI.A. S.R.L., Italy) and [...]
Evaluation of Cytotoxicity of Perfluorocarbons for Intraocular Use by Cytotoxicity Test In Vitro in Cell Lines
Year: 2019 Authors: Romano R. M.; Ferrara M.; Gatto C.; Ferrari B.; Giurgola L.; D’Amato Tóthová J. Translational Vision Science & Technology: October 2019, Vol.8, 24 doi:10.1167/tvst.8.5.24 Online version This is a: Publication Purpose: To validate the cytotoxicity test of perfluorocarbon liquids (PFCLs) for intraocular use according to the ISO 10993-5 standard. Methods: BALB/3T3, ARPE-19 [...]
Antimycotic efficacy and safety of a new cold corneal storage medium by time–kill and toxicity studies
Year: 2019 Authors: Giurgola L.; Gatto C.; Parel J.M.; Miller D.; DʼAmato Tóthová J. Cornea. 2019 Oct;38(10):1314-1321. doi: 10.1097/ICO.0000000000002068 Online version This is a: Publication Purpose: To evaluate a new corneal cold storage medium including an antimycotic tablet (Kerasave, AL.CHI.MI.A. S.r.l.). Methods: Kerasave and tryptone soy broth (control) were inoculated with 10 and 10 colony-forming [...]
H content is not predictive of perfluorocarbon ocular endo-tamponades cytotoxicity in vitro
Year: 2019 Authors: Ruzza P.; Gatto C.; Ragazzi E.; Romano R. M.; Honisch C.; D’Amato Tóthová J. ACS Omega Journal: August 2, 2019 doi.org/10.1021/acsomega.9b01793 Online version This is a: Publication Abstract: In recent years, cases of retinal toxicity occurred in some European, Middle Eastern, and South American countries following the use of perfluorocarbon liquids (PFCLs) on vitreoretinal surgeries owing to [...]
Efficacy and Safety of Various Amphotericin B Concentrations on Candida albicans in Cold Storage Conditions
Year: 2019 Authors: K. D. Tran, PhD; B. T. Aldrich, PhD; J. D’Amato Tóthová, PhD; J. M. Skeie, PhD; C. M. Kondratick, PhD; L. Giurgola, MSc; C. Gatto, MSc, C. R. Reed, RN, PhD; G. A. Schmidt, BS, CEBT; M. A. Terry, MD; M. A. Greiner, MD Cornea: June 13, 2019 doi: 10.1097/ICO.0000000000002019 Online version This is a: [...]
A two-centre validation study of sterility test of corneal storage media with elimination of interfering antimicrobials in compliance with the European Pharmacopoeia
Year: 2019 Authors: Mistò R.; Giurgola L.; Pateri F.; Frigerio E.; Limongelli A.; D’Amato Tóthová J. Cell Tissue Bank (2019) 20: 275 doi.org/10.1007/s10561-019-09766-7 Online version This is a: Publication The purpose of this study was to validate the sterility test of corneal culture and deswelling/transport media using a device for removal of [...]
Increased sensitivity of microbiological testing of cornea organ culture medium by additional resin treatment
Year: 2018 Authors: Skenderi Z.; Giurgola L.; Gatto C.; D'Amato Tóthová J.; Pruß A.; Schroeter J. Category: Microbiology BMJ Open Opthalmology 2018;3:e000173. doi:10.1136/bmjophth-2018-000173 Online version This is a: Publication Objective: This validation study investigates the treatment of cornea organ culture medium (Modified Eagle Medium, Biochrom GmbH, Berlin, Germany) with RESEP, a new medical device for antibiotics [...]

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OUR VISION

NEW PROJECTS

Cornea Surgery protectants

Retina surgery staining

Vitreous visualization

Retinal surgery instruments

Cryoprotectants

Innovative storage media

Post-surgery eye drops

EXPERTISE

SCIENTIFIC COLLABORATIONS

The latest R&D project

OPHTHALMIC DIAGNOSTICS

ON MARKET

RINSING

BASE

DECONTAMINATION

BASE.128

CRYOPRESERVATION

CRYO.ON

CRYOPRESERVATION

GLYO.ON

OPHTHALMIC GEL

eyeDRO

DYES FOR POSTERIOR SEGMENT

TWIN

DYES FOR POSTERIOR SEGMENT

viewILM

MICROBIOLOGY

RESEP

METHODS

SCIENTIFIC HIGHLIGHTS

PUBLICATIONS

Title

Ophthalmic Surgery

Human Tissue Banking

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